Evidence for New Transcriptional Units Encoded at the 3′ End of the Mouse Hepatitis Virus Genome
Identifieur interne : 001350 ( Main/Exploration ); précédent : 001349; suivant : 001351Evidence for New Transcriptional Units Encoded at the 3′ End of the Mouse Hepatitis Virus Genome
Auteurs : Mary C. Schaad [États-Unis] ; Ralph S. Baric [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1993.
Abstract
Abstract: Prior studies have demonstrated that the mouse hepatitis virus (MHV) genome is divided into at least seven coding regions each transcribed into a distinct mRNA. The majority of these mRNAs are synthesized from a highly conserved intergenic start site (UCU/CAAAC), contain a 65-72 nt leader RNA at their 5′ end and form a 3′ co-terminal nested set. In this study, we have used radiolabeling experiments to demonstrate the presence of a small ∼900 nt mRNA and its corresponding RF RNA in MHV-infected cells. Surprisingly, PCR amplification and sequence analysis revealed the presence of not one, but two small leader-containing RNAs that initiate from highly conserved intergenic start sites (UCCAAAC and UCUAAAU) which are located within the 3′-most nucleocapsid gene sequence. These studies provide evidence suggesting that one or two additional small mRNAs are encoded from the 3′ end of the MHV genome.
Url:
DOI: 10.1006/viro.1993.1467
Affiliations:
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